736 Defining the epigenetic regulation of fibroblast lineages during embryonic development

The mammalian skin can support hair follicle formation during embryonic development, but this ability is lost as the matures. We have previously shown that, in murine starting at day 16.5 (E16.5), dermal fibroblasts contained two distinct lineages with different functions. upper papillary population supports of new follicles. However, mechanism by which are established remained undefined. By performing ChIP-seq for histone modifications markers comparing early (E14.5) and late differentiated (E17.5), we found a significant difference H3K27me3 peaks genes associated fibroblast differentiation. performed vivo reconstitution assays multi-omics experiments conjunction tissue specific knockout models to define epigenetic mechanisms that regulate heterogeneity dermis.